Bulletin of the IDF N° 478/ 2015: Interlaboratory collaborative study on a flow cytometry method for lactic acid bacteria quantification in starter cultures, probiotics and fermented milk products according to ISO 19344/IDF 232

Bulletin of the IDF No. 478/2015

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Product Description

An international collaborative study of ISO 19344│IDF 232, Milk and milk products

– Starter cultures, probiotics and fermented milk products – Quantification of lactic

acid bacteria by flow cytometry was conducted to determine precision figures for

the quantification of lactic acid bacteria and probiotic strains by flow cytometry in

starter cultures, probiotics and fermented milk products. Except for yoghurts, the

repeatability limit (r) and reproducibility limit (R) values were calculated to be 0.06

and 0.45 for log10 active fluorescent units (AFU) per gram and 0.07 and 0.38 for log10

total fluorescent units (TFU) per gram. The corresponding values for yoghurts were

0.08 and 0.76 for log10 AFU per gram and 0.10 and 1.17 for log10 TFU per gram. The

latter reproducibility value illustrates the challenges encountered in analysis of TFU

in yoghurts. Furthermore, equivalence of AFU results between the three different

staining protocols was evaluated and demonstrated for most of the strains included in

this study.

Keywords: Starter cultures, Probiotics, Lactic acid bacteria, Fermented milk, Flow

cytometry, Collaborative study, Precision figures

Additional Information

Language

EN

Reference

Bulletin of the IDF No. 478/2015

Date

2015

Type

Hard Copy, PDF

Pages

48